| Title | Season | Episode # | First Aired |
|---|---|---|---|
| Diego's Wolf Pup Rescue | 2 | 21 | Monday September 18, 2006 |
| Diego Saves Baby River Dolphin | 2 | 22 | Monday October 2, 2006 |
| Diego and Baby Humpback to the Rescue | 2 | 23 | Tuesday October 3, 2006 |
| Little Kinkajou Is in Beehive Trouble | 2 | 24 | Wednesday October 4, 2006 |
| Macky the Macaroni Penguin | 2 | 25 | Thursday October 5, 2006 |
| The Iguana Sing Along | 2 | 26 | Sunday November 19, 2006 |
| Panchita Prairie Dog Finds a New Prairie | 2 | 27 | Sunday December 3, 2006 |
| Diego Saves Christmas | 2 | 28 | Friday December 8, 2006 |
| The Great Jaguar Rescue | 2 | 29 | Monday January 15, 2007 |
| Diego the Hero | 2 | 30 | Monday February 5, 2007 |
| Sammy's Valentine | 2 | 31 | Wednesday February 14, 2007 |
| The Tapir's Trip Home | 2 | 32 | Tuesday March 6, 2007 |
| Jorge the Little Hawk Learns to Migrate | 2 | 33 | Wednesday March 7, 2007 |
| Giant Octopus to the Rescue | 2 | 34 | Thursday March 8, 2007 |
| The Great Roadrunner Race | 2 | 35 | Monday March 26, 2007 |
| An Underwater Mystery | 2 | 36 | Tuesday June 26, 2007 |
| Rainforest Rhapsody | 2 | 37 | Wednesday September 5, 2007 |
| Gorilla Fun | 2 | 38 | Thursday September 6, 2007 |
| Diego's African Safari | 2 | 39 | Monday October 8, 2007 |
| Diego and Alicia Save the Otters | 2 | 40 | Tuesday October 16, 2007 |
| Alicia Saves the Crocodile | 2 | 41 | Saturday November 24, 2007 |
| Rhea Is an Animal Rescuer | 2 | 42 | Wednesday December 19, 2007 |